DOI: https://doi.org/10.36719/2663-4619/127/269-276
Mahruh Nagiyeva 
Azerbaijan State Agricultural University
Ganja, Azerbaijan
PhD in Pharmacy
mahrux.nagiyeva@adau.edu.az
Aytakin Mammadova
Azerbaijan State Agricultural University
Ganja, Azerbaijan
PhD in Pharmacy
aytakin.mammadova@adau.edu.az
Shafiga Jafarova
Azerbaijan State Agricultural University
Ganja, Azerbaijan
shafiqa.cafarova@adau.edu.az
Ilaha Shirinova
Azerbaijan State Agricultural University
Ganja, Azerbaijan
ilaha.shirinova@adau.edu.az
Huseyn Kazimzada
Azerbaijan State Agricultural University
Ganja, Azerbaijan
huseyn.kazimzada@adau.edu.az
Quantification and Validation of Flavonoids in Astrantia Maxima Pall. Using Spectrophotometry
Abstract
This study aimed to quantify the flavonoid content in Astrantia maxima Pall. raw material using a spectrophotometric approach and to assess the method’s validation parameters. Flowering-stage raw material of Astrantia maxima Pall. was collected in June 2025 from the Dashkasan district, Republic of Azerbaijan. Spectrophotometric determination of flavonoid content was performed, and method validation including accuracy, precision, and linearity was conducted following international regulatory guidelines. The total flavonoid content in Astrantia maxima Pall. was found to be 1.01% per gram of raw material. Validation outcomes indicated high reliability: accuracy demonstrated a confidence interval of 0.51; linearity was established over a concentration range of 0.01–0.06 mg/mL (R² = 0.9989); precision exhibited coefficients of variation of 0.0005%, 0.0006%, and 0.0006% across three consecutive days. These results confirm that Astrantia maxima Pall. is a rich source of flavonoids, highlighting its potential for incorporation into local medicinal plant-based resources and further pharmacological studies.
Keywords: Astrantia maxima Pall., flavonoids, spectrophotometric analysis, method validation, raw material