Genetic Diversity Analysis of Diospyros Genotypes From Azerbaijan Using Scot Molecular Markers
Natavan Bakhshaliyeva
Abstract. The Diospyros genus contains approximately 400 to 500 species, among which D. kaki is cultivated as the edible fruit crop. Assessement of genetic diversity within Diospyros genotypes is essential for breeding and conservation programs. In the present study, genetic diversity among six Diospyros genotypes was evaluated using Start Codon Targeted (SCOT) molecular markers. PCR amplification with the SCOT 28 primer generated clear and reproducible banding patterns. A total of 10 bands were detected, of which 8 were polymorphic, resulting in 80% polymorphism. The polymorphic information content (PIC) ranged from 0.28 to 0.50 with an average value of 0.41. The resolving power (Rp) was estimated at 5.6, indicating good discriminatory ability of the primer. Genetic similarity analysis based on Jaccard coefficient revealed moderate variation among genotypes, confirming the presence of genetic diversity. The results demonstrate that SCOT markers are effective tools for assessing genetic diversity and can be applied in Diospyros germplasm characterization, conservation strategies, and future breeding programs.
Keywords: SCOT marker, Diospyros, genetic diversity, polymorphism, PIC, resolving power