DOI: https://doi.org/10.36719/2707-1146/61/11-16
Samira Hummatova
Baku State University
Candidate of Biological Sciences
https://orcid.org/0009-0000-7774-9552
sam_bio@mail.ru
Natella Kocharli
Baku State University
PhD in Biology
https://orcid.org/0009-0009-9943-1559
natella.kocharli@gmail.om
Chemiluminescence Study of the Formation of Protein Radicals Under the Influence of Ultraviolet-B Rays
Abstract
The presented work reviews the molecular mechanisms of the formation of reactive oxygen species (ROS) in protein solutions exposed to ultraviolet-B (UV-B) (mainly UV-B: 280–315 nm) radiation, the role of proteins in their formation, and the biological significance of these processes. It is known that ROS species, in turn, cause the formation of long-lived protein radicals (LLPRs). The amount of LLPRs formed in human serum albumin protein upon exposure to UV-B (1.2×10², 2.4×10², 3.6×10², and 4.8×10² erg/mm²) irradiation was studied by the chemiluminescence (XL) method. It was observed that the intensity of XL varies depending on the irradiation dose. The introduction of antioxidants into the system leads to a weakening of the effect of UV radiation. Thus, inosine neutralizes long-lived protein radicals more effectively than natural antioxidants. In our opinion, since ROS play a signaling and regulatory role in biological studies, their formation and LLPRs play an important role in the adaptation of living systems to stress factors.
Keywords: Long-lived protein radicals, oxidative stress, reactive oxygen species, UV-B radiation, chemiluminescence